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KMID : 0812020140200010041
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Korean Journal of Neurogastroenterology and Motility
2014 Volume.20 No. 1 p.41 ~ p.53
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Dietary Glutamate: Interactions With the Enteric Nervous System
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Wang Guo-Du
Xia Yun
Wang Xi-Yu
Wood Jackie D
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Abstract
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Background/Aims: Digestion of dietary protein elevates intraluminal concentrations of glutamate in the small intestine, some of which gain access to the enteric nervous system (ENS). Glutamate, in the central nervous system (CNS), is an excitatory neurotransmitter. A dog-ma that glutamatergic neurophysiology in the ENS recapitulates CNS glutamatergic function persists. We reassessed the prem-ise that glutamatergic signaling in the ENS recapitulates its neurotransmitter role in the CNS.
Methods: Pharmacological analysis of actions of receptor agonists and antagonists in concert with immunohistochemical localization of glutamate transporters and receptors was used. Analysis focused on intracellularly-recorded electrical and synaptic behavior of ENS neurons, on stimulation of mucosal secretion by secretomotor neurons in the submucosal plexus and on muscle con-tractile behavior mediated by musculomotor neurons in the myenteric plexus.
Results: Immunoreactivity for glutamate was expressed in ENS neurons. ENS neurons expressed immunoreactivity for the EAAC-1 gluta-mate transporter. Neither L-glutamate nor glutamatergic receptor agonists had excitatory actions on ENS neurons. Metabotropic glutamatergic receptor agonists did not directly stimulate neurogenic mucosal chloride secretion. Neither L-gluta-mate nor the metabotropic glutamatergic receptor agonist, aminocyclopentane-1,3-dicarboxylic acid (ACPD), changed the mean amplitude of spontaneously occurring contractions in circular or longitudinal strips of intestinal wall from either guinea pig or human small intestinal preparations.
Conclusions: Early discoveries, for excitatory glutamatergic neurotransmission in the CNS, inspired enthusiasm that investigation in the ENS would yield discoveries recapitulating the CNS glutamatergic story. We found this not to be the case.
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KEYWORD
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Intestines, Motility, Proteolysis, Receptors, glutamate, Secretion
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